Brain-derived neurotrophic factors (BDNF), which regulate the survival and growth of neurons and influence synaptic efficiency, plasticity and other aspects of synaptic function, is known as brain-derived neurotrophic force. There are 11 exons in the human BDNF gene. Different BDNF transcripts can be produced by using different promoters or splicing events.
Although the majority of BDNF transcripts are found in the brain, as well as in blood cells and other tissues, no studies have yet examined the differential expression of BDNF transcripts in peripheral tissues. You can buy BDNF ELISA Kit via online sources. This ELISA kit employs the Sandwich-ELISA principle.
This kit includes a micro ELISA plate that has been pre-coated in an antibody specific for Mouse BDNF. The specific antibody is added to the micro ELISA plates wells by adding samples or standards. After that, a biotinylated detection antibody specific for Mouse BDNF conjugate is added to each microplate and incubated. All components that are not required for the experiment are removed.
Each well is treated with the substrate solution. Blue color will only be seen in those wells that have Avidin-HRP conjugate, biotinylated antibody and Mouse BDNF. Add a stop solution to the enzyme-substrate mixture and the color will turn yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +- 2 nm.
The concentration of Mouse BDNF is proportional to its optical density (OD). The standard curve can be used to calculate the concentration of Mouse-BDNF in samples. BDNF belongs to the neurotrophin family of growth factors that are closely related to NGF.